Validated Spectrophotometric method for analysis of Dipyridamole and Lamivudine using eosin Y

Barghash, Sona Soliman; Abd El-Razeq, Sawsan; El -Awady, Mohamed; Belal, Fathalla

doi:10.21608/aijpms.2021.52761.1016

Validated Spectrophotometric method for analysis of Dipyridamole and Lamivudine using eosin Y

Document Type : Original research articles

Authors

¹ Pharmaceutical Analytical Chemistry ,Faculty of Pharmacy,Al-Azhar University ,Cairo,Egypt

² Analytical Chemistry,Faculty of Pharmacy,Al-Azhar University,Cairo,Egypt

³ Analytical Chemistry,Faculty of Pharmacy,Mansoura University,Mansoura, Egypt

⁴ Analytical Chemistry,Faculty of Pharmacy,Mansoura University,Mansoura,Egypt

10.21608/aijpms.2021.52761.1016

Abstract

Validated, accurate, simple and reliable spectrophotometric method was described for the assay of dipyridamole and lamivudine in their different dosage forms. The suggested method depended on a binary reddish orange coloured complexformation between eosin Y and each one of the two analytes in presence of carboxy methyl cellulose and aqueous buffered medium for dipyridamole and lamivudine, respectively at pH 3.7. The coloured binary complexes exhibited maximum absorbance at 554.0 nm for dipyridamole and lamivudine. The absorbance concentration graphs were linear over ranges of (1.0-10.0 μg/mL) and (2.0-20.0 μg/mL), with lower detection limits of 0.27 μg/mL and 0.46 μg/mL and lower quantitation limits of 0.82 μg/mL and 1.40 μg/mL for dipyridamole and lamivudine, respectively. The developed method was applicable to the assay of dipyridamole and lamivudine in their different dosage forms, and the obtained results were in acceptable agreement with all those provided by reference methods. The present method is appropriate for the assay of the two drugs on account of its cost effectiveness, simplicity and rapidity

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