UPLC-MS/MS profiling and antitumor activity of Silene succulenta Forssk. Growing in Egypt

Document Type : Original research articles

Authors

1 Department of Medicinal and Aromatic plants, Desert Research Center, Cairo, Egypt

2 Department of Pharmacognosy, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

3 Department of Pharmacognosy, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt

Abstract

Abstract
The present study aims to detect the phenolics and flavonoids constituents of Silene succulenta Forssk. belonging to Caryophyllaceae family, also screening of their antioxidant and cytotoxic activities. GC-MS analysis is one of our goals in this study. The phenolic and flavonoid profiles were carried out using Micro plate assay. In addition, in vitro antioxidant activity was performed using DPPH assay while the Cytotoxicity was summarized by SRB test. GC-MS analysis for n-hexane was carried out using gas chromatography-mass spectrometry. Also ultra-performance liquid chromatography coupled with hybrid triple time-of-flight mass spectrometry (UPLC-Triple TOF-MS/MS) was carried out to determine the different phytochemicals in total alcoholic extract. Total phenolic and flavonoids content in aerial parts of S. succulenta were found 20.86 mg/g and 8.06 mg/g respectively. Cytotoxicity of n-hexane fraction showed a high activity against Breast (MCF7) and Lung Carcinoma (A549) cell lines with IC50 15.5 and 22.6 µg /ml respectively. Total alcoholic extract and ethyl acetate fraction showed antioxidant activities with IC50 799.8 ± 45.45 and 749.9 ± 29.73 (ug/ ml) respectively. Finally, ninety six compounds were detected in n-hexane using GC-MS and comparison with standards. UPLC-Triple TOF-MS/MS showed the presence of different classes of secondary metabolites (flavonoids, coumarins, amino acids, anthocyanidines and alkaloids)

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