Cytogenetic Effect of Heavy Metal Exposure in Alzheimer’s Disease Rat Model

Document Type : Original research articles

Authors

1 Biochemistry department, Faculty of Pharmacy , October 6 University

2 Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Girls), Azhar University

3 Department of Genetic and Cytology, Division of Genetic Engineering and Biotechnology, National Research Centre, Dokki, Gizza, Egypt

Abstract

The widespread heavy metal, potassium dichromate (PDC), presents an actual threat because of its high toxicity to all body organs. This research was designed to study the possible cytotoxicity and genotoxicity exerted by PDC on Alzheimer’s disease (AD) and the prospect negative effect on AD induced in rats. The current research was performed on forty male rats, classified similarly into 4 sets: normal control, AD control was done by intraperitoneal injection of lipopolysaccharide (LPS) at a dose 250 µg/kg body weight for 7 days, AD intraperitoneally injected once by PDC 10 mg/kg body weight, and PDC-intoxicated rats (same as the third group). The forth group served to compare the effect of PDC alone. Twenty-four hours after the PDC injection, rats were sacrificed to collect their brain hippocampi (CA3 region) to study its histopathological structure, acetylcholinesterase (AChE) gene expression by RT-PCR, malondialdehyde (MDA) content and catalase (CAT) activity spectrophotometrically. Additionally, cells of the bone marrow were harvested from the rat femoral bone to undergo in-vivo micronucleus (MN) assay. The outcomes entailed that AD rats with PDC significantly altered the hippocampal histology, vanished neuronal survival count, increased AChE gene expression, MDA content and CAT activity in AD rats more than normal and PDC-only rats. Furthermore, PDC significantly elevated frequency of MN in AD rats’ bone marrow cells. In conclusion, the study results evidenced that PDC exerted a tremendous harm to the AD case, evident by all previously mentioned outcomes.

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Azhar International Journal of Pharmaceutical and Medical Sciences
Volume 1, Issue 3
The articles belongs to the special issue selected papers from Azhar 2nd International Conference of Pharmaceutical and Medical Sciences (2021)
November 2021
Pages 56-65

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