Document Type : Original research articles
Authors
1
Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University (Girls), Cairo , Egypt
2
Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University (Boys), Cairo11884 , Egypt.
3
Department of Pharmacognosy, Faculty of Pharmacy, Sinai University - Kantara Branch, Ismailia 41636, Egypt.
4
Department of Biochemistry, Egyptian Drug Authority (EDA), Formerly National Organization for Drug Control and Research (NODCAR), Giza, Egypt.
5
Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University (Girls), Cairo, Egypt.
Abstract
Strelitzia reginae is used in some African countries as a traditional medicine with very few
phytochemical and biological assessments. The study aims to isolate and structure elucidate of S. reginae
phytoconstituents using different chromatographic and spectral techniques. In addition to, antimicrobial
assessment of ethyl acetate and n-butanol fractions on both clinical isolates and standard strains. Strelitzia
reginae belongs to the family Strelitziaceae which is a rich source of biologically active phytoconstituents and
has diverse therapeutic values. Strelitzia reginae leaves were fractionated into ethyl acetate and n-butanol, and
five compounds were detected. Compounds were identified as kaempferol (1), quercetin (2), daucosterol (3),
vitexin (4), and rutin (5). The isolated compounds were elucidated by their NMR and ESI-MS spectral data.
Antimicrobial activity of Strelitzia reginae ethyl acetate and n-butanol fractions screened against six different
bacterial strains (three Gram-positive, three Gram-negative, and one fungus isolate). Both fractions showed
antimicrobial activities against Klebsiella pneumonia and Staphylococcus aureus which is methicillinresistant (MRSA) strains. As well, the activity of antimicrobial was detected against Proteus mirabilis clinical
isolate from urine and MRSA. On the other hand, both fractions showed inadequate antimicrobial activities
against the Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans clinical isolates compared
to reference drugs. The antimicrobial results were in accordance with previously reported folk uses and
attributed to the isolated phytoconstituents.
Keywords
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